Inter Milan's standout teenage defender Davide Santon has got his first call-up by Italy Under-21 coach Pierluigi Casiraghi for the friendly against Netherlands in Kerkrade on March 31.
Juventus defender Claudio Marchisio returns after a long injury and Lorenzo Ariaudo, who scored one of the two goals in a friendly against Austria on Wednesday, has been confirmed.
Inter's starting striker Mario Balotelli and Juventus winger Sebastian Giovinco will be expected to star up front.
The Netherlands game will be the penultimate test for the Azzurrini before the final phase of the European championship.
The last friendly will be on June 9 against Denmark.
Italy has won the European under-21 championships five times since 1992.
Netherlands have won the last two.
Here is the squad: Goalies: Consigli (Atalanta), Sirigu (Ancona); Defenders: Ariaudo (Juventus), Criscito (Genoa), De Silvestri (Lazio), Marzoratti (Empoli), Ranocchia (Bari), Santacroce (Napoli), Santon (Inter); Midfielders: Candreva (Livorno), Cigarini (Atalanta), Dessena (Sampdoria), Marchisio (Juventus), Morosini (Vicenza), Poli (Sasssuolo); Forwards: Acquafresca (Cagliari), Balotelli (Inter), Di Gennaro (Reggina), Giovinco (Juventus).